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Article|01 Jun 2021|OPEN
A critical role of PvFtsH2 in the degradation of photodamaged D1 protein in common bean
Kun Xu1, Hong Zhai1, Hongyan Wu1, Yi Gao1,2, Yuzhuo Li2,1, Xiaobin Zhu1,2, Jinlong Zhu1 & Zhengjun Xia1,
1Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Haping Road 138, Nangang District, 150081 Harbin City, Heilongjiang Province, China
2University of Chinese Academy of Sciences, Beijing, China

Horticulture Research 8,
Article number: 126 (2021)
doi: 10.1038/hortres.2021.126
Views: 426

Received: 02 Nov 2020
Revised: 13 Mar 2021
Accepted: 22 Mar 2021
Published online: 01 Jun 2021

Abstract

Light is required for initiating chloroplast biogenesis and photosynthesis; however, the photosystem II reaction center (PSII RC) can be photodamaged. In this study, we characterized pvsl1, a seedling-lethal mutant of Phaseolus vulgaris. This mutant showed lethality when exposed to sunlight irradiation and a yellow-green leaf phenotype when grown in a growth chamber under low-light conditions. We developed 124 insertion/deletion (INDEL) markers based on resequencing data of Dalong1 and PI60234, two local Chinese common bean cultivars, for genetic mapping. We identified Phvul.002G190900, which encodes the PvFtsH2 protein, as the candidate gene for this pvsl1 mutation through fine-mapping and functional analysis. A single-base deletion occurred in the coding region of Phvul.002G190900 in the pvsl1 mutant, resulting in a frameshift mutation and a truncated protein lacking the Zn2+ metalloprotease domain. Suppressed expression of Phvul.002G190900 at the transcriptional level was detected, while no change in the subcellular localization signal was observed. The seedlings of pvsl1 exhibited hypersensitivity to photoinhibition stress. In the pvsl1 mutant, abnormal accumulation of the D1 protein indicated a failure to rapidly degrade damaged D1 protein in the PSII RC. The results of this study demonstrated that PvFtsH2 is critically required for survival and maintaining photosynthetic activity by degrading photodamaged PSII RC D1 protein in common bean.