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Article|01 May 2021|OPEN
Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola
Guo-Tian Liu1,2,3, Bian-Bian Wang1,2, David Lecourieux3, Mei-Jie Li1,2, Ming-Bo Liu1,2, Rui-Qi Liu1,2, Bo-Xing Shang1,2, Xiao Yin1,2, Li-Jun Wang4, Yan Xu1,2, & Fatma Lecourieux3,
1State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, China
2Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, China
3 UMR1287 EGFV, CNRS, Université de Bordeaux, INRAE, Bordeaux Sciences Agro, ISVV, Villenave d’Ornon, France
4 Institute of Botany, The Chinese Academy of Sciences, Beijing, China

Horticulture Research 8,
Article number: 100 (2021)
doi: 10.1038/hortres.2021.100
Views: 165

Received: 15 Jun 2020
Revised: 16 Jan 2021
Accepted: 24 Feb 2021
Published online: 01 May 2021

Abstract

Wild grapevines can show strong resistance to the downy mildew pathogen P. viticola, but the associated mechanisms are poorly described, especially at early stages of infection. Here, we performed comparative proteomic analyses of grapevine leaves from the resistant genotype V. davidii “LiuBa-8” (LB) and susceptible V. vinifera “Pinot Noir” (PN) 12 h after inoculation with P. viticola. By employing the iTRAQ technique, a total of 444 and 349 differentially expressed proteins (DEPs) were identified in LB and PN, respectively. The majority of these DEPs were related to photosynthesis, respiration, cell wall modification, protein metabolism, stress, and redox homeostasis. Compared with PN, LB showed fewer downregulated proteins associated with photosynthesis and more upregulated proteins associated with metabolism. At least a subset of PR proteins (PR10.2 and PR10.3) was upregulated upon inoculation in both genotypes, whereas HSP (HSP70.2 and HSP90.6) and cell wall-related XTH and BXL1 proteins were specifically upregulated in LB and PN, respectively. In the incompatible interaction, ROS signaling was evident by the accumulation of H2O2, and multiple APX and GST proteins were upregulated. These DEPs may play crucial roles in the grapevine response to downy mildew. Our results provide new insights into molecular events associated with downy mildew resistance in grapevine, which may be exploited to develop novel protection strategies against this disease.