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Article|01 May 2021|OPEN
Comparative transcriptomic analysis highlights contrasting levels of resistance of Vitis vinifera and Vitis amurensis to Botrytis cinerea
Ran Wan1,2, Chunlei Guo1,3, Xiaoqing Hou1,4, Yanxun Zhu1,4, Min Gao1,4, Xiaoyan Hu1,3, Songlin Zhang1,4, Chen Jiao1,5, Rongrong Guo1,6, Zhi Li1,4 & Xiping Wang1,4,
1State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, 712100 Yangling, Xianyang, Shaanxi, China
2College of Horticulture, Henan Agricultural University, 450002 Zhengzhou, Henan, China
3College of Horticulture Science and Technology, Hebei Normal University of Science and Technology, 066004 Qinhuangdao, Hebei, China
4Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, 712100 Yangling, Xianyang, Shaanxi, China
5Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853, USA
6Grape and Wine Research Institute, Guangxi Academy of Agricultural Sciences, 53000 Nanning, Guangxi, China

Horticulture Research 8,
Article number: 103 (2021)
doi: 10.1038/hortres.2021.103
Views: 201

Received: 24 May 2020
Revised: 23 Feb 2021
Accepted: 08 Mar 2021
Published online: 01 May 2021

Abstract

Botrytis cinerea is a major grapevine (Vitis spp.) pathogen, but some genotypes differ in their degree of resistance. For example, the Vitis vinifera cultivar Red Globe (RG) is highly susceptible, but V. amurensis Rupr Shuangyou (SY) is highly resistant. Here, we used RNA sequencing analysis to characterize the transcriptome responses of these two genotypes to B. cinerea inoculation at an early infection stage. Approximately a quarter of the genes in RG presented significant changes in transcript levels during infection, the number of which was greater than that in the SY leaves. The genes differentially expressed between infected leaves of SY and RG included those associated with cell surface structure, oxidation, cell death and C/N metabolism. We found evidence that an imbalance in the levels of reactive oxygen species (ROS) and redox homeostasis probably contributed to the susceptibility of RG to B. cinerea. SY leaves had strong antioxidant capacities and improved ROS homeostasis following infection. Regulatory network prediction suggested that WRKY and MYB transcription factors are associated with the abscisic acid pathway. Weighted gene correlation network analysis highlighted preinfection features of SY that might contribute to its increased resistance. Moreover, overexpression of VaWRKY10 in Arabidopsis thaliana and V. vinifera Thompson Seedless enhanced resistance to B. cinerea. Collectively, our study provides a high-resolution view of the transcriptional changes of grapevine in response to B. cinerea infection and novel insights into the underlying resistance mechanisms.