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Article|10 Jan 2024|OPEN
Chloroplast C-to-U editing, regulated by a PPR protein BoYgl-2, is important for chlorophyll biosynthesis in cabbage 
Bin Zhang1,2 ,† , Yuankang Wu1,2 ,† , Shoufan Li2 ,† , Wenjing Ren1,2 , Limei Yang2 , Mu Zhuang2 , Honghao Lv2 and Yong Wang2 , Jialei Ji2 , Xilin Hou1 , , Yangyong Zhang,2 ,
1State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
2State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
*Corresponding author. E-mail: hxl@njau.edu.cn,zhangyangyong@caas.cn
†Bin Zhang and Yuankang Wu,Shoufan Li contributed equally to the study.

Horticulture Research 11,
Article number: uhae006 (2024)
doi: https://doi.org/10.1093/hr/uhae006
Views: 37

Received: 12 Oct 2023
Accepted: 30 Dec 2023
Published online: 10 Jan 2024

Abstract

Leaf color is an important agronomic trait in cabbage (Brassica oleracea L. var. capitata), but the detailed mechanism underlying leaf color formation remains unclear. In this study, we characterized a Brassica oleracea yellow-green leaf 2 (BoYgl-2) mutant 4036Y, which has significantly reduced chlorophyll content and abnormal chloroplasts during early leaf development. Genetic analysis revealed that the yellow-green leaf trait is controlled by a single recessive gene. Map-based cloning revealed that BoYgl-2 encodes a novel nuclear-targeted P-type PPR protein, which is absent in the 4036Y mutant. Functional complementation showed that BoYgl-2 from the normal-green leaf 4036G can rescue the yellow-green leaf phenotype of 4036Y. The C-to-U editing efficiency and expression levels of atpF, rps14, petL and ndhD were significantly reduced in 4036Y than that in 4036G, and significantly increased in BoYgl-2 overexpression lines than that in 4036Y. The expression levels of many plastid- and nuclear-encoded genes associated with chloroplast development in BoYgl-2 mutant were also significantly altered. These results suggest that BoYgl-2 participates in chloroplast C-to-U editing and development, which provides rare insight into the molecular mechanism underlying leaf color formation in cabbage.